A Non-Radioactive Method for Measuring PP2A Activity in Plants

A Non-Radioactive Method for Measuring PP2A Activity in Plants

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Protein 15-eg2373cl phosphatase 2A (PP2A) is a group of important cellular regulators in eukaryotes that dephosphorylate more than 30% of cellular proteins whose activities are turned on or off by phosphorylation.In plants, PP2A was found to regulate critical components involved in plant growth and development, and in response to biotic and abiotic stresses.Therefore, determining the PP2A activities at different developmental stages, in different tissues, or in various mutants is critical in order to understand the functions of PP2A in plants.Traditional PP2A enzyme assay uses moen finney radioactive isotope and often take days to finish.This PP2A enzyme assay described here is a method to determine PP2A activity without using radioactive materials in less than 6 h.